5JMQ

Crystal Structure of Mus musculus Protein Arginine Methyltransferase 2 with CP3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	65.700, 114.685, 132.159
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.309 - 1.795
R-factor	0.1803
Rwork	0.179
R-free	0.20140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fub
RMSD bond length	0.006
RMSD bond angle	0.878
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_1980)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.050	1.830
High resolution limit [Å]	1.790	1.790
Rmerge	0.113	1.957
Number of reflections	46366
<I/σ(I)>	9.8	0.7
Completeness [%]	98.7	84.9
Redundancy	6.5	5.1
CC(1/2)	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	22% PEG6000, 100mM CaCl2, 100mM HEPES pH7.0