5JMG
X-ray structure of the complex between bovine pancreatic ribonuclease and pentachlorocarbonyliridate(III) (4 days of soaking)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-16 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.932, 32.652, 72.273 |
| Unit cell angles | 90.00, 90.28, 90.00 |
Refinement procedure
| Resolution | 72.270 - 1.850 |
| R-factor | 0.1754 |
| Rwork | 0.173 |
| R-free | 0.22580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jvt |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.373 |
| Data reduction software | CrystalClear |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.270 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.063 | |
| Number of reflections | 19871 | |
| <I/σ(I)> | 2.15 | 2.3 |
| Completeness [%] | 97.8 | 93.5 |
| Redundancy | 2.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 293 | 22% PEG4K, 10 mM sodium citrate pH 5.1 |
