5JMD

Heparinase III-BT4657 gene product, Methylated Lysines

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-01
Detector	RAYONIX MX-300
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	66.359, 80.412, 83.543
Unit cell angles	90.00, 104.08, 90.00

Refinement procedure

Resolution	40.517 - 2.400
R-factor	0.2206
Rwork	0.219
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.583
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.517
High resolution limit [Å]	2.400
Number of reflections	33448
<I/σ(I)>	9.3
Completeness [%]	99.5
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	25% PEG 3350, 100mM Tris pH8.5, 200mM magnesium chloride and 500mM NDSB 201 additive