5JM6

Structure of Chaetomium thermophilum mApe1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	P 2 21 21
Unit cell lengths	121.019, 143.894, 201.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.073 - 2.758
R-factor	0.2093
Rwork	0.208
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vat
RMSD bond length	0.014
RMSD bond angle	1.908
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((dev_2313: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.700	2.800
High resolution limit [Å]	2.750	2.750
Rmerge	0.130	0.770
Number of reflections	172990
<I/σ(I)>	0.98	1.3
Completeness [%]	98.7	93.5
Redundancy	3.1	3
CC(1/2)	0.980	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	277	PROTEIN BUFFER: 2.0-3.0 mg/ml in 50 mM Tris-HCl pH 7.5, 50 mM NaCl; RESERVOIR BUFFER: 100 mM Hepes, pH 6.6, 4 M sodium formate