5JLX
AntpHD with 15bp DNA duplex S-monothioated at Cytidine-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-31 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 60.864, 75.581, 91.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.666 - 2.748 |
| R-factor | 0.1954 |
| Rwork | 0.193 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xid |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.786 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.748 | 2.750 |
| Rmerge | 0.152 | 1.302 |
| Number of reflections | 11412 | |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 6.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 20mM bis-Tris propane, 10mM NiCl2, 4-6% 2-methyl-2,4-pentanediol |
