5JLX

AntpHD with 15bp DNA duplex S-monothioated at Cytidine-8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-31
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	P 2 2 21
Unit cell lengths	60.864, 75.581, 91.876
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.666 - 2.748
R-factor	0.1954
Rwork	0.193
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xid
RMSD bond length	0.007
RMSD bond angle	0.786
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.748	2.750
Rmerge	0.152	1.302
Number of reflections	11412
<I/σ(I)>	4.4
Completeness [%]	99.4	99.1
Redundancy	6.4	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	20mM bis-Tris propane, 10mM NiCl2, 4-6% 2-methyl-2,4-pentanediol