5JLT

The crystal structure of the bacteriophage T4 MotA C-terminal domain in complex with dsDNA reveals a novel protein-DNA recognition motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-25
Detector	MAR CCD 130 mm
Wavelength(s)	1.0000
Spacegroup name	P 61
Unit cell lengths	72.270, 72.270, 279.369
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.588 - 2.955
R-factor	0.2208
Rwork	0.220
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1KAF + DNA helix from COOT
RMSD bond length	0.007
RMSD bond angle	1.544
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((dev_2363))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.120	3.110
High resolution limit [Å]	2.955	2.955
Rmerge	0.167
Number of reflections	17332
<I/σ(I)>	15.1	2
Completeness [%]	100.0	100
Redundancy	23.4	23.5
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	23% PEG 8K, 0.1 M Na Acetate, 0.1 M NaCacodylate, pH 6.5, and 3% glycerol