5JLT
The crystal structure of the bacteriophage T4 MotA C-terminal domain in complex with dsDNA reveals a novel protein-DNA recognition motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-25 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | P 61 |
Unit cell lengths | 72.270, 72.270, 279.369 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 62.588 - 2.955 |
R-factor | 0.2208 |
Rwork | 0.220 |
R-free | 0.24460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1KAF + DNA helix from COOT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.544 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2363)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.120 | 3.110 |
High resolution limit [Å] | 2.955 | 2.955 |
Rmerge | 0.167 | |
Number of reflections | 17332 | |
<I/σ(I)> | 15.1 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 23.4 | 23.5 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 23% PEG 8K, 0.1 M Na Acetate, 0.1 M NaCacodylate, pH 6.5, and 3% glycerol |