5JLF
Structure of the F-actin-tropomyosin complex (Reprocessed)
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Number of Particle | 119000 |
| Software | SPARX,Coot,REFMAC,RELION,EPU,CTFFIND,UCSF Chimera,iMODFIT |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
