5JLD
Crystal Structure of Arginyl-tRNA Synthetase from Plasmodium falciparum (PfRRS)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97903 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.196, 62.079, 114.640 |
Unit cell angles | 90.00, 96.05, 90.00 |
Refinement procedure
Resolution | 41.987 - 2.200 |
R-factor | 0.1874 |
Rwork | 0.184 |
R-free | 0.24480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4q2y |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
Rmerge | 0.059 | 0.034 | 0.557 |
Number of reflections | 32304 | ||
<I/σ(I)> | 7.7 | ||
Completeness [%] | 98.4 | 97.3 | 97.8 |
Redundancy | 4.6 | 4.4 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10.0%(W/V) PEG6000, 2.0%(W/V) PEG3350, 5%(V/V) MPD, 0.1M HEPES pH 7.5 |