5JLA

Crystal Structure of Ribose-5-phosphate Isomerase from Brucella melitensis 16M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	126.480, 109.960, 64.990
Unit cell angles	90.00, 93.90, 90.00

Refinement procedure

Resolution	41.934 - 1.450
R-factor	0.2066
Rwork	0.206
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3awd
RMSD bond length	0.007
RMSD bond angle	0.959
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MoRDa
Refinement software	PHENIX (dev_2356)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.934
High resolution limit [Å]	1.450
Number of reflections	154161
<I/σ(I)>	10.67
Completeness [%]	98.6
Redundancy	4.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	290	MCSG-1 c6(269929c6): 30% PPG P400, 0.2M MgCl, 0.1M Hepes, pH=7.5; cryo: direct; BuxeA.00010.g.B1.ps02594 at 7.5 mg/ml, puck rpa3-9