5JKT
vaccinia virus D4 P173G mutant /A20(1-50)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 107 |
| Detector technology | PIXEL |
| Collection date | 2014-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.980, 92.980, 145.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.610 - 2.490 |
| R-factor | 0.18562 |
| Rwork | 0.183 |
| R-free | 0.23772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oda |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.989 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.610 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 26149 | |
| <I/σ(I)> | 19.7 | 3.8 |
| Completeness [%] | 99.5 | 96 |
| Redundancy | 9.5 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 293 | 0.1M bicine pH 8.9, 1.6M ammonium sulphate |
