5JKR
vaccinia virus D4/A20(1-50)w43a mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 107 |
Detector technology | PIXEL |
Collection date | 2014-02-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 92.980, 92.980, 145.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.510 - 2.600 |
R-factor | 0.198 |
Rwork | 0.195 |
R-free | 0.24969 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4oda |
RMSD bond length | 0.015 |
RMSD bond angle | 1.848 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.510 | 2.710 |
High resolution limit [Å] | 2.590 | 2.590 |
Number of reflections | 23077 | |
<I/σ(I)> | 16.1 | 3.2 |
Completeness [%] | 99.4 | 95.5 |
Redundancy | 12.9 | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 293 | 0.1M bicine pH 8.9, 1.5M ammonium sulphate |