5JKC

Crystal structure of human IZUMO1-JUNO complex (crystal form 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-26
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	65.188, 144.750, 141.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.900
R-factor	0.2273
Rwork	0.225
R-free	0.25941
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jk9 5jka
RMSD bond length	0.012
RMSD bond angle	1.648
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.900
Number of reflections	15228
<I/σ(I)>	34.5
Completeness [%]	100.0
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	8-12% PEG4000, 0.2 M Li2SO4, 0.1 M MES-NaOH pH 6.0