5JKB
Crystal structure of human JUNO (crystal form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-22 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 96.770, 88.579, 108.132 |
| Unit cell angles | 90.00, 96.88, 90.00 |
Refinement procedure
| Resolution | 44.300 - 3.230 |
| R-factor | 0.21773 |
| Rwork | 0.216 |
| R-free | 0.24053 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jka |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.443 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.300 |
| High resolution limit [Å] | 3.230 |
| Number of reflections | 14574 |
| <I/σ(I)> | 8 |
| Completeness [%] | 99.4 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3% (w/v) PEG4000, 5% 2-propanol, 0.1 M HEPES-NaOH pH 7.5 |
