5JKA
Crystal structure of human JUNO (crystal form 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-22 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 51.920, 81.038, 235.084 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.19855 |
Rwork | 0.197 |
R-free | 0.21782 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kmy |
RMSD bond length | 0.010 |
RMSD bond angle | 1.476 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 33749 |
<I/σ(I)> | 26.6 |
Completeness [%] | 99.2 |
Redundancy | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG3350, 0.24 M malonate pH 7.0 |