5JK6

Phenylalanine hydroxylase from dictyostelium - apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.23984
Spacegroup name	P 1 21 1
Unit cell lengths	70.871, 85.309, 74.913
Unit cell angles	90.00, 110.04, 90.00

Refinement procedure

Resolution	37.498 - 2.072
R-factor	0.1615
Rwork	0.159
R-free	0.20770
RMSD bond length	0.007
RMSD bond angle	1.010
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.070
Rmerge	0.100
Number of reflections	49361
<I/σ(I)>	9.6
Completeness [%]	96.7
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	26%(w/v) PEG 2000, 0.06 M PIPES,