5JK4
Phosphate-Binding Protein from Stenotrophomonas maltophilia.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.729, 77.856, 56.287 |
| Unit cell angles | 90.00, 102.08, 90.00 |
Refinement procedure
| Resolution | 38.928 - 1.100 |
| R-factor | 0.1026 |
| Rwork | 0.102 |
| R-free | 0.12460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q9t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.961 |
| Data reduction software | DIALS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.940 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.076 | 0.631 |
| Number of reflections | 120995 | |
| <I/σ(I)> | 9.7 | 2.3 |
| Completeness [%] | 94.1 | 85.9 |
| Redundancy | 4.4 | 3.7 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 15 % w/v PEG 8K, 100 mM TRIS pH 8.0, 10 mM sodium formate. |
