5JJZ

Chromo domain of human Chromodomain Protein, Y-Like 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97912
Spacegroup name	P 61 2 2
Unit cell lengths	57.962, 57.962, 98.824
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.010 - 2.000
R-factor	0.2315
Rwork	0.230
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hae
RMSD bond length	0.011
RMSD bond angle	1.393
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	MOLREP (11.0.05)
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.051	0.030	0.845
Total number of observations	153213
Number of reflections	7130
<I/σ(I)>	15.5
Completeness [%]	99.6	95.7	98.8
Redundancy	21.5	17.4	16.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2 M K/Na tartrate