5JJW
Crystal structure of the HAT domain of sart3 in complex with USP15 DUSP-UBL domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 117.925, 117.925, 205.326 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.670 - 3.010 |
| R-factor | 0.2367 |
| Rwork | 0.234 |
| R-free | 0.28830 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
| Rmerge | 0.197 | 0.058 | 2.594 |
| Rmeas | 0.201 | ||
| Rpim | 0.036 | ||
| Total number of observations | 539429 | ||
| Number of reflections | 17447 | ||
| <I/σ(I)> | 4 | ||
| Completeness [%] | 100.0 | 99.4 | 100 |
| Redundancy | 30.9 | 26.5 | 31.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 19% PEG 3350, 0.2 M MgCl2, 0.1 M HEPES pH7.5, 5% Ethylene Glycol |
