5JJQ
Crystal structure of IdnL1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 65.750, 104.450, 120.000 |
Unit cell angles | 81.78, 83.01, 81.98 |
Refinement procedure
Resolution | 59.510 - 2.600 |
R-factor | 0.23532 |
Rwork | 0.234 |
R-free | 0.26517 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jjp |
RMSD bond length | 0.014 |
RMSD bond angle | 1.704 |
Data reduction software | iMOSFLM (1.0.7) |
Data scaling software | SCALA (3.3.20) |
Phasing software | MOLREP (11.0.05) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.510 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.080 | 0.307 |
Number of reflections | 90052 | |
<I/σ(I)> | 6.3 | 2 |
Completeness [%] | 94.1 | 94.6 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | PEG3350, sodium citrate, potassium chloride, ATP, magnesium chloride, (S)-3-aminobutyrate, Tris, glycerol |