5JJN

Structure of the SRII/HtrII(G83F) Complex in P212121 space group ("V" shape)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	47.131, 109.096, 121.818
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.055 - 2.250
R-factor	0.1883
Rwork	0.186
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hs2
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.055	2.370
High resolution limit [Å]	2.250	2.250
Rmerge	0.123	0.501
Number of reflections	30445
<I/σ(I)>	14.3
Completeness [%]	99.5
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	1 M Na/K phosphate pH 5.6