5JJJ

Structure of the SRII/HtrII Complex in P64 space group ("U" shape)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	P 64
Unit cell lengths	66.310, 66.310, 170.081
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.000 - 2.500
R-factor	0.1819
Rwork	0.179
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hs2
RMSD bond length	0.008
RMSD bond angle	1.292
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.090	0.396
Number of reflections	20997
<I/σ(I)>	11.16
Completeness [%]	77.6
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	1 M Na/K phosphate pH 5.6