5JJE

Structure of the SRII/HtrII Complex in I212121 space group ("U" shape)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-20
Detector	ADSC QUANTUM 210
Wavelength(s)	0.987
Spacegroup name	I 21 21 21
Unit cell lengths	49.642, 113.661, 125.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.135 - 1.900
R-factor	0.1978
Rwork	0.197
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2s
RMSD bond length	0.011
RMSD bond angle	1.233
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.135	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.070	0.393
Number of reflections	28430
<I/σ(I)>	13.4
Completeness [%]	99.4
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	1 M Na/K phosphate, pH 5.6