5JJ2
Crystal structure of the central domain of human AKAP18 gamma/delta in complex with malonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.250, 60.260, 65.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.360 - 1.250 |
| R-factor | 0.1618 |
| Rwork | 0.161 |
| R-free | 0.18030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vfy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.850 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX (Version 1.10-2152) |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.360 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.043 | |
| Number of reflections | 119131 | |
| <I/σ(I)> | 18.5 | 1.49 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 12.1 | 10.7 |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.5 M Sodium malonate |
