5JIP
Crystal structure of the Clostridium perfringens spore cortex lytic enzyme SleM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-05 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.599, 85.852, 87.205 |
Unit cell angles | 90.00, 105.51, 90.00 |
Refinement procedure
Resolution | 42.397 - 1.800 |
R-factor | 0.1526 |
Rwork | 0.151 |
R-free | 0.18210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jfx |
RMSD bond length | 0.010 |
RMSD bond angle | 1.065 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2313: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.400 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.357 | |
Number of reflections | 66317 | |
<I/σ(I)> | 24.5 | 5 |
Completeness [%] | 99.6 | 98.2 |
Redundancy | 3.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | 0.1 M MES buffer, pH 6.0, 0.25 M magnesium chloride, 16% (w/v) PEG6000 |