5JIG
Crytsal structure of Wss1 from S. pombe
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-09-23 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.8 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.300, 41.300, 68.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.000 |
R-factor | 0.14265 |
Rwork | 0.141 |
R-free | 0.16724 |
Structure solution method | SAD |
RMSD bond length | 0.021 |
RMSD bond angle | 1.913 |
Data reduction software | XDS (20151015) |
Data scaling software | XDS (20151015) |
Phasing software | SHARP (2.8.5) |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.100 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.059 | 0.399 |
Number of reflections | 61720 | |
<I/σ(I)> | 21 | 4.9 |
Completeness [%] | 98.7 | 99.7 |
Redundancy | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM Tris/HCl, 20% PEG 1000 |