5JIA

The Crystal Structure Of IUS-SPRY Domain From RanBP10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-20
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	146.340, 79.240, 157.630
Unit cell angles	90.00, 97.91, 90.00

Refinement procedure

Resolution	41.250 - 1.800
R-factor	0.184
Rwork	0.184
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ji7
RMSD bond length	0.007
RMSD bond angle	1.194
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.081	0.272
Number of reflections	330519
<I/σ(I)>	14.159	2.563
Completeness [%]	92.6	85.2
Redundancy	4.1	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	295	0.1M BICINE, PH 9.0, 10% PEG 20000, 0- 2% DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K