5JI7

The Crystal Structure Of IUS-SPRY Domain From RanBPM/9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	42.697, 62.666, 69.766
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.420 - 1.510
R-factor	0.165
Rwork	0.163
R-free	0.19100
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.251
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.560
High resolution limit [Å]	1.510	1.510
Rmerge	0.048	0.154
Number of reflections	30090
<I/σ(I)>	44.825	10.683
Completeness [%]	99.2	93.7
Redundancy	8.4	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.1M NA-CACODYLATE, PH 6.5, 6% PEG 4000, 0.2M KCL, 0.02M MGCL2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.1M NA-CACODYLATE, PH 6.5, 6% PEG 4000, 0.2M KCL, 0.02M MGCL2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K