5JHT

Apo form of influenza strain H1N1 polymerase acidic subunit N-terminal region crystallized with potassium sodium tartrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	66.859, 66.859, 128.003
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.345 - 1.751
R-factor	0.1965
Rwork	0.194
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zqq
RMSD bond length	0.017
RMSD bond angle	1.634
Data scaling software	HKL-2000
Phasing software	MOLREP (11.0.05)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.059	1.359
Number of reflections	29953
<I/σ(I)>	44.219	2.34
Completeness [%]	99.6	100
Redundancy	14	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.1 M MES pH 6.0, 0.8 M Potassium sodium tartrate