5JHQ
ARCs 1-3 of human Tankyrase-1 bound to a peptide derived from IRAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.510, 129.830, 123.950 |
| Unit cell angles | 90.00, 92.31, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.21514 |
| Rwork | 0.214 |
| R-free | 0.23947 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tww |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.301 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 48511 |
| <I/σ(I)> | 11.8 |
| Completeness [%] | 98.2 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 1.8 M (NH4)2SO4 100 mM Tris pH 8.5 |
