5JHL
Crystal structure of zika virus envelope protein in complex with a flavivirus broadly-protective antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.600, 104.856, 81.862 |
| Unit cell angles | 90.00, 102.70, 90.00 |
Refinement procedure
| Resolution | 30.693 - 3.000 |
| R-factor | 0.2298 |
| Rwork | 0.228 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gms |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.928 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 16275 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 98.6 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M sodium chloride, 0.1 Na/K phosphate, pH 6.5, 25 % PEG 1000 |
