5JHE
The Crystal Structure of the Saccharomyces cerevisiae Co-Chaperone Cpr7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 42 |
Unit cell lengths | 75.332, 75.332, 100.794 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.920 - 1.800 |
R-factor | 0.18597 |
Rwork | 0.185 |
R-free | 0.21082 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ihg |
RMSD bond length | 0.009 |
RMSD bond angle | 1.463 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.149 |
Number of reflections | 51730 |
<I/σ(I)> | 6.7 |
Completeness [%] | 98.4 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 12% (w/v) PEG 8000, 0.1M Ca acetate, 0.1M Na cacodylate, pH 5.5 |