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5JHE

The Crystal Structure of the Saccharomyces cerevisiae Co-Chaperone Cpr7

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRF BEAMLINE BL19U1
Synchrotron siteSSRF
BeamlineBL19U1
Temperature [K]100
Detector technologyCCD
Collection date2015-09-28
DetectorADSC QUANTUM 315r
Wavelength(s)0.9792
Spacegroup nameP 42
Unit cell lengths75.332, 75.332, 100.794
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution41.920 - 1.800
R-factor0.18597
Rwork0.185
R-free0.21082
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ihg
RMSD bond length0.009
RMSD bond angle1.463
Data reduction softwareHKL-3000
Data scaling softwareHKL-3000
Phasing softwareMOLREP
Refinement softwareREFMAC (5.7.0032)
Data quality characteristics
 Overall
Low resolution limit [Å]50.000
High resolution limit [Å]1.800
Rmerge0.149
Number of reflections51730
<I/σ(I)>6.7
Completeness [%]98.4
Redundancy3.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.528912% (w/v) PEG 8000, 0.1M Ca acetate, 0.1M Na cacodylate, pH 5.5

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