5JHC
Crystal structure of the self-assembled propeptides from Ape1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2015-05-16 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 248.758, 248.758, 164.403 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.519 - 3.400 |
R-factor | 0.2781 |
Rwork | 0.276 |
R-free | 0.31090 |
Structure solution method | SIRAS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.292 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 3.460 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 26941 | |
<I/σ(I)> | 22.4 | 4.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.3 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 500 mM magnesium sulfate, 100 mM sodium acetate |