5JH1
Crystal structure of the apo form of AKR4C7 from maize
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.458 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.220, 55.978, 103.258 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.950 - 1.450 |
R-factor | 0.1339 |
Rwork | 0.132 |
R-free | 0.17600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eko |
RMSD bond length | 0.017 |
RMSD bond angle | 1.682 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.691 | 49.211 | 1.530 |
High resolution limit [Å] | 1.450 | 4.590 | 1.450 |
Rmerge | 0.073 | 0.071 | 0.412 |
Number of reflections | 49709 | ||
<I/σ(I)> | 13.8 | 7.9 | 1.8 |
Completeness [%] | 98.8 | 99.6 | 97.1 |
Redundancy | 5.2 | 9.6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 0,1 M Tris-HCl, pH, 6.0, 22% m/v PEG4000 and 0,1 M sodium acetate |