5JGZ
Spin-Labeled T4 Lysozyme Construct T151V1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-11 |
Detector | RIGAKU |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.460, 59.460, 95.302 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.730 - 1.534 |
R-factor | 0.1693 |
Rwork | 0.167 |
R-free | 0.19540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c6t |
RMSD bond length | 0.006 |
RMSD bond angle | 0.741 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.980 | |
High resolution limit [Å] | 1.534 | 1.534 |
Number of reflections | 29805 | |
<I/σ(I)> | 11.46 | |
Completeness [%] | 100.0 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium/potassium phosphate, sodium chloride, hexane-1,6-diol, 2-propanol |