5JGX

Spin-Labeled T4 Lysozyme Construct V131V1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-11
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	60.103, 60.103, 96.551
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.050 - 1.533
R-factor	0.1616
Rwork	0.160
R-free	0.18890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c6t
RMSD bond length	0.006
RMSD bond angle	0.813
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.050
High resolution limit [Å]	1.533	1.533
Number of reflections	30915
<I/σ(I)>	24.26
Completeness [%]	100.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	293	sodium/potassium phosphate, sodium chloride, hexane-1,6-diol, 2-propanol