5JGR
Spin-Labeled T4 Lysozyme Construct K43V1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.760, 59.760, 96.259 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.750 - 1.460 |
R-factor | 0.1679 |
Rwork | 0.166 |
R-free | 0.19280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c6t |
RMSD bond length | 0.010 |
RMSD bond angle | 0.769 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.750 | |
High resolution limit [Å] | 1.460 | 1.460 |
Number of reflections | 34628 | |
<I/σ(I)> | 6.3 | |
Completeness [%] | 98.0 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 297 | sodium/potassium phosphate, sodium chloride, hexane-1,6-diol, 2-propanol |