5JGE
Crystal structure of Atg19 coiled-coil complexed with Ape1 propeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-02-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000, 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.875, 32.921, 56.957 |
| Unit cell angles | 87.19, 76.52, 67.10 |
Refinement procedure
| Resolution | 55.330 - 1.910 |
| R-factor | 0.19698 |
| Rwork | 0.194 |
| R-free | 0.24615 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.633 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.330 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.458 |
| Number of reflections | 13677 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 97.4 | 96.2 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30% (w/v) PEG 4000, 200 mM sodium acetate, 100 mM Tris-HCl |
