5JGB

Crystal structure of human TAK1/TAB1 fusion protein in complex with ligand 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-31
Detector	MARRESEARCH
Wavelength(s)	1.0000
Spacegroup name	I 2 2 2
Unit cell lengths	58.711, 134.970, 143.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.580 - 2.800
R-factor	0.2026
Rwork	0.201
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2eva
RMSD bond length	0.008
RMSD bond angle	1.206
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	98.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.103	0.037	0.456
Number of reflections	13809
<I/σ(I)>	8.4
Completeness [%]	95.7	97.3	50.4
Redundancy	3.8	3.7	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	293	1.7M sodium potassium phosphate, 20%(v/v) Glycerol as cryoprotectant