5JG9
Crystal structure of the de novo mini protein gEHEE_06
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-08 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.920, 45.533, 49.704 |
| Unit cell angles | 90.00, 105.09, 90.00 |
Refinement procedure
| Resolution | 47.990 - 2.090 |
| R-factor | 0.20306 |
| Rwork | 0.201 |
| R-free | 0.25084 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.199 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.090 |
| Rmerge | 0.111 |
| Number of reflections | 8734 |
| <I/σ(I)> | 14.4 |
| Completeness [%] | 96.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | Peg, buffer 7 |
