5JFV
Crystal structure of TrkA in complex with PF-05206283
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 64 |
Unit cell lengths | 124.540, 124.540, 46.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.280 - 1.590 |
R-factor | 0.1727 |
Rwork | 0.172 |
R-free | 0.19150 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.9.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.850 | 1.670 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.475 | |
Number of reflections | 54128 | |
<I/σ(I)> | 24.3 | 2 |
Completeness [%] | 96.5 | 79.2 |
Redundancy | 8.3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | protein was concentrated to 10mg/ml. Crystals of complexes were grown using the hanging drop vapour diffusion method from 0.1M HEPES pH7.5, 0.1M MgCl2 and 10% EtOH at 4C |