5JFB
Crystal structure of the scavenger receptor cysteine-rich domain 5 (SRCR5) from porcine CD163
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2015-09-14 |
| Detector | Pilatus |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 29.711, 73.930, 87.527 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.760 - 2.000 |
| R-factor | 0.20922 |
| Rwork | 0.208 |
| R-free | 0.23905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1by2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.276 |
| Data scaling software | HKL-3000 |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.107 | |
| Number of reflections | 6682 | |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 97.1 | |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 25% (w/v) PEG 4000, 200mM (NH4)2SO4 in 100mM sodium acetate at pH 4.6 |
