5JEY
Crystal structure of type 2 PDF from Streptococcus agalactiae, crystallized in cacodylate buffer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.360, 65.980, 89.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.530 - 2.800 |
| R-factor | 0.18535 |
| Rwork | 0.182 |
| R-free | 0.24787 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aie |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.606 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.480 | |
| Number of reflections | 11619 | |
| <I/σ(I)> | 8.8 | 3.43 |
| Completeness [%] | 98.0 | 93.2 |
| Redundancy | 2.55 | 2.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 9% PEG-8000, 100mM Na Cacodylate pH6.5, 100mM Zn acetate |
