5JEY

Crystal structure of type 2 PDF from Streptococcus agalactiae, crystallized in cacodylate buffer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	41.360, 65.980, 89.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.530 - 2.800
R-factor	0.18535
Rwork	0.182
R-free	0.24787
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aie
RMSD bond length	0.030
RMSD bond angle	2.606
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.780	2.780
Rmerge		0.480
Number of reflections	11619
<I/σ(I)>	8.8	3.43
Completeness [%]	98.0	93.2
Redundancy	2.55	2.44

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	9% PEG-8000, 100mM Na Cacodylate pH6.5, 100mM Zn acetate