5JEX
Crystal structure of type 2 PDF from Streptococcus agalactiae, crystallized in imidazole buffer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9755 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.320, 65.550, 88.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.370 - 2.000 |
| R-factor | 0.16116 |
| Rwork | 0.159 |
| R-free | 0.20774 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aie |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.557 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 16886 | |
| <I/σ(I)> | 17.04 | 6.63 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 7% PEG-8000 , 100mM Imidazole pH7.5, 300mM Zn Acetate |
