5JEQ
Fragment of nitrate/nitrite sensor histidine kinase NarQ (R50K) in symmetric apo state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.972 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 39.593, 59.366, 239.583 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.640 - 1.900 |
R-factor | 0.23424 |
Rwork | 0.232 |
R-free | 0.28498 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iji |
RMSD bond length | 0.007 |
RMSD bond angle | 1.018 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.640 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.067 | 1.230 |
Number of reflections | 22889 | |
<I/σ(I)> | 13.77 | 1.42 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.42 | 6.72 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | in meso lipidic cubic phase crystallization |