5JEN

Crystal structure of the anti-sigma factor RsiV bound to lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2014-04-30
Detector	RDI CMOS_8M
Wavelength(s)	0.979
Spacegroup name	P 2 21 21
Unit cell lengths	45.213, 130.326, 136.712
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.166 - 2.300
R-factor	0.1837
Rwork	0.181
R-free	0.23660
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.159
Data reduction software	XDS (VERSION January 10, 2014)
Data scaling software	XDS (VERSION January 10, 2014)
Phasing software	PHENIX (1.10_2155)
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.530	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.109	0.899
Number of reflections	36905
<I/σ(I)>	14	2.6
Completeness [%]	98.9	99.9
Redundancy	7.2	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M sodium nitrate, 20% (w/v) PEG 3350.