5JEF
Fragment of nitrate/nitrite sensor histidine kinase NarQ (WT) in asymmetric holo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 53.464, 39.865, 120.361 |
| Unit cell angles | 90.00, 100.62, 90.00 |
Refinement procedure
| Resolution | 52.500 - 2.420 |
| R-factor | 0.22087 |
| Rwork | 0.218 |
| R-free | 0.28569 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iji |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.882 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.500 | 2.500 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.101 | 0.832 |
| Number of reflections | 18994 | |
| <I/σ(I)> | 7.86 | 1.62 |
| Completeness [%] | 97.4 | 89.8 |
| Redundancy | 3.34 | 3.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | in meso lipidic cubic phase crystallization |
