5JDK

Crystal structure of the DNA binding domain of Sap1 in fission yeast S.pombe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	36.840, 40.880, 70.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.870 - 0.998
R-factor	0.1699
Rwork	0.170
R-free	0.17860
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.911
Data reduction software	HKL-2000 (0.98.691)
Data scaling software	CrystalClear (1.44)
Phasing software	PHENIX (1.10.1)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.870	1.034
High resolution limit [Å]	0.998	0.998
Rmerge	0.049	0.598
Number of reflections	58240
<I/σ(I)>	6.88	1.22
Completeness [%]	99.9	98.6
Redundancy	6.6	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	293	200mM Trimethylamine N-oxide, 15-22% (w/v) PEG 2000MME
1	VAPOR DIFFUSION, SITTING DROP	8.8	293	200mM Trimethylamine N-oxide, 15-22% (w/v) PEG 2000MME