5JDJ

Crystal structure of domain I10 from titin in space group P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9173
Spacegroup name	P 21 21 21
Unit cell lengths	74.637, 136.239, 140.095
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.652 - 1.738
R-factor	0.1864
Rwork	0.186
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.115
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.652	1.800
High resolution limit [Å]	1.738	1.738
Number of reflections	145378
<I/σ(I)>	15.38	2.77
Completeness [%]	98.8	95.19
Redundancy	7.5	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.2 M CaCl2, 0.1 M Tris pH 7.5, 25% [w/v] PEG 8000