5JDB
Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2015-11-14 |
Detector | AGILENT EOS CCD |
Wavelength(s) | 0.97944 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.428, 114.931, 73.581 |
Unit cell angles | 90.00, 96.05, 90.00 |
Refinement procedure
Resolution | 28.980 - 1.901 |
R-factor | 0.2036 |
Rwork | 0.202 |
R-free | 0.22960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dwr |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 83986 |
<I/σ(I)> | 17 |
Completeness [%] | 97.6 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2.0 M ammonium sulfate |