5JD2
SFX structure of corestreptavidin-selenobiotin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2015-07-17 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.840, 98.520, 53.430 |
| Unit cell angles | 90.00, 112.38, 90.00 |
Refinement procedure
| Resolution | 32.506 - 1.900 |
| R-factor | 0.1658 |
| Rwork | 0.165 |
| R-free | 0.19950 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.030 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | DM |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.510 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 75562 |
| <I/σ(I)> | 14 |
| Completeness [%] | 100.0 |
| Redundancy | 1447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | 24% PEG 1500 and 20% glycerol |
